Microbial community of healthy Thai vegetarians and non-vegetarians, their core gut microbiota and pathogens risk.

Ruengsomwong S1,2,3, La-Ongkham O1, Jiang J4, Wannissorn B3, Nakayama J4, Nitisinprasert S1,2.

Abstract


Pyrosequencing analysis of intestinal microflora from healthy Thai vegetarians and non-vegetarians exhibited 893 OTUs covering 189 species. The strong species indicator of vegetarians and non-vegetarians were Prevotella copri and Bacteroides vulgatus as well as bacterium closed toEscherichia hermanii with % relative abundance of 16.9 and 4.5-4.7, respectively. Core gut microbiota of vegetarian and non-vegetarian group consisted of 11 and 20 different bacterial species, respectively, belonging to Actinobacteria, Firmicutes and Proteobacteria commonly found in both groups. Two species of Faecalibacterium prausnitzii and Gemmiger formicilis had prevalence of 100% in both groups. Three species ofClostridium nexile, Eubacterium eligens and P. copri showed up in most vegetarians while more diversity of Collinsella aerofaciens,Ruminococcus torques, various species of Bacteroides, Parabacteroides, Escherichia, different species of Clostridium and Eubacterium were found in most non-vegetarians. Considering the correlation of personal characters, consumption behavior and microbial groups, the age of non-vegetarians showed strong positive correlation coefficient of 0.54 (p=0.001) to Bacteroides uniformis while exhibited a moderate ones to Alistipes finegoldii and B. vulgatus. Only positive moderate correlation of body mass index (BMI) and Parabacteroides distasonis appeared. Based on significant abundance of potential pathogens, the microbiota of non-vegetarian group showed the abundance of potential pathogen varieties ofBilophila wadsworthia, Escherichia coli and E. hermannii while the one of vegetarian served for only Klebsiella pneumonia. These results implied that the microbiota of vegetarian with high abundance of P. copri and low potential pathogen variety would be a way to maintain healthy in Thai.

KEYWORDS:

Enterobacteriaceae; Prevotella; core gut microbiota; fecal microbiota; pathogen risk; pyrosequencing
PMID:
 
27381339
 
DOI:
 
10.4014/jmb.1603.03057                                                                                                                                            
 2016 Jul 6. doi: 10.4014/jmb.1603.03057